| 质粒名称: | 1.3xWT HBV-Luciferase |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 61549 |
| 存储实验室: | Yosef Shaul |
|
载体骨架基本信息 |
|
| 载体名称: | pGEM3Z |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 1.3 times of the HBV genome with luciferase within the Core ORF |
| 物种来源: | -- |
| 插入大小: | 3932 bp |
| 基因突变: | 用荧光素酶ORF替换HBV序列。没有载体哺乳动物启动子。HBV固有增强子和启动子 |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | 1.3xWT HBV-Luciferase was a gift from Yosef Shaul (Addgene plasmid # 61549 ; http://n2t.net/addgene:61549 ; RRID:Addgene_61549) |
| 参考文献部分: | Enhancer I predominance in hepatitis B virus gene expression. Doitsh G, Shaul Y. Mol Cell Biol. 2004 Feb;24(4):1799-808. PubMed 14749394 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 1.3xWT HBV-Luciferase 6656 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION The 1.3xHBV-Luciferase plasmid to monitor HBV transcription. 1.
Luciferase inserted within Core ORF. 2. Luciferase is regulated by
EnhII - Core promoter. 3. The functional luciferase ATG is of Core.
ACCESSION .
VERSION .
KEYWORDS 1.3xWT HBV-Luciferase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6656)
AUTHORS Doitsh G, Shaul Y
TITLE Enhancer I predominance in hepatitis B virus gene expression.
JOURNAL Mol Cell Biol. 2004 Feb;24(4):1799-808.
PUBMED 14749394
REFERENCE 2 (bases 1 to 6656)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6656
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 972..2621
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(1035..1053)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
promoter complement(3981..3999)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3982..3999)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4017..4033)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4017..4033)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4030..4052)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4041..4057
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4065..4095)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4110..4131
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4248..4265)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4419..5007)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4499..4518)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5178..6038)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5801..5820
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6039..6143)
/gene="bla"
/label=AmpR promoter
primer_bind 6211..6229
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6267..6289)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6389..6408
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6602..6624
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6616..6633
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6617..6633
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6640..3
/label=T7
/note="T7 promoter, forward primer"
promoter 6640..2
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gggcgaattc gagctcggta cccatcctgc cttaatgcct ttgtatgcat gtatacaagc
61 taaacaggct ttcactttct cgccaactta caaggccttt ctaagtaaac agtacatgaa
121 cctttacccc gttgctcggc aacggcctgg tctgtgccaa gtgtttgctg acgcaacccc
181 cactggctgg ggcttggcca taggccatca gcgcatgcgt ggaacctttg tggctcctct
241 gccgatccat actgcggaac tcctagccgc ttgttttgct cgcagccggt ctggagcaaa
301 gctcatcgga actgacaatt ctgtcgtcct ctcgcggaaa tatacatcgt ttccatggct
361 gctaggctgt actgccaact ggatccttcg cgggacgtcc tttgtttacg tcccgtcggc
421 gctgaatccc gcggacgacc cctcgcgggg ccgcttggga ctctctcgtc cccttctccg
481 tctgccgttc cagccgacca cggggcgcac ctctctttac gcggtctccc cgtctgtgcc
541 ttctcatctg ccggtccgtg tgcacttcgc ttcacctctg cacgttgcat ggagaccacc
601 gtgaacgccc atcagatcct gcccaaggtc ttacataaga ggactcttgg actcccagca
661 atgtcaacga ccgaccttga ggcctacttc aaagactgtg tgtttaagga ctgggaggag
721 ctgggggagg agattaggtt aaaggtcttt gtattaggag gctgtaggca taaattggtc
781 tgcgcaccag caccatgcaa ctttttcacc tctgcctaat catctcttgt acatgtccca
841 ctgttcaagc ctccaagctg tgccttgggt ggctttgggg catggacatt gacccttata
901 aagaatttgg agctactgtg gagttactct cgtttttgcc ttctgacttc tttccttccg
961 tcagagatct cgaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
1021 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
1081 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
1141 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
1201 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
1261 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
1321 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
1381 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
1441 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
1501 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
1561 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
1621 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
1681 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
1741 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
1801 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
1861 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
1921 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
1981 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
2041 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
2101 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
2161 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
2221 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
2281 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
2341 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
2401 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
2461 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
2521 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
2581 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta aactagtgcc atttgttcag
2641 tggttcgtag ggctttcccc cactgtttgg ctttcagcta tatggatgat gtggtattgg
2701 gggccaagtc tgtacagcat cgtgagtccc tttataccgc tgttaccaat tttcttttgt
2761 ctctgggtat acatttaaac cctaacaaaa caaaaagatg gggttattcc ctaaacttca
2821 tgggctacat aattggaagt tggggaactt tgccacagga tcatattgta caaaagatca
2881 aacactgttt tagaaaactt cctgttaaca ggcctattga ttggaaagta tgtcaaagaa
2941 ttgtgggtct tttgggcttt gctgctccat ttacacaatg tggatatcct gccttaatgc
3001 ctttgtatgc atgtatacaa gctaaacagg ctttcacttt ctcgccaact tacaaggcct
3061 ttctaagtaa acagtacatg aacctttacc ccgttgctcg gcaacggcct ggtctgtgcc
3121 aagtgtttgc tgacgcaacc cccactggct ggggcttggc cataggccat cagcgcatgc
3181 gtggaacctt tgtggctcct ctgccgatcc atactgcgga actcctagcc gcttgttttg
3241 ctcgcagccg gtctggagca aagctcatcg gaactgacaa ttctgtcgtc ctctcgcgga
3301 aatatacatc gtttccatgg ctgctaggct gtactgccaa ctggatcctt cgcgggacgt
3361 cctttgttta cgtcccgtcg gcgctgaatc ccgcggacga cccctctcgg ggccgcttgg
3421 gactctctcg tccccttctc cgtctgccgt tccagccgac cacggggcgc acctctcttt
3481 acgcggtctc cccgtctgtg ccttctcatc tgccggtccg tgtgcacttc gcttcacctc
3541 tgcacgttgc atggagacca ccgtgaacgc ccatcagatc ctgcccaagg tcttacataa
3601 gaggactctt ggactcccag caatgtcaac gaccgacctt gaggcctact tcaaagactg
3661 tgtgtttaag gactgggagg agctggggga ggagattagg ttaaaggtct ttgtattagg
3721 aggctgtagg cacaaattgg tctgcgcacc agcaccatgc aactttttca cctctgccta
3781 atcatctctt gtacatgtcc cactgttcaa gcctccaagc tgtgccttgg gtggctttgg
3841 ggcatggaca ttgaccctta taaagaattt ggagctactg tggagttact ctcgtttttg
3901 ccttctgact tctttccttc cgtcagagat ctcctagaca ccgcctcagc tctgtattgc
3961 aggcatgcaa gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
4021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
4081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
4141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
4201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
4261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
4321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
4381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
4441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
4501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
4561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
4621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
4681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
4741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
4801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
4861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
4921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
4981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
5041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
5101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
5161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
5221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
5281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
5341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
5401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
5461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
5521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
5581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
5641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
5701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
5761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
5821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
5881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
5941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
6001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
6061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
6121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
6181 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
6241 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
6301 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
6361 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
6421 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
6481 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
6541 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
6601 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//
